Wool Fibres: Sectioning and Staining, Differentiation of Ortho and Paracortex
January 1965
in “
Stain Technology
”
Studysummary This study describes a double embedding technique for preparing tangential sections of hair and wool fibers, recommending specific staining methods for quantitative analysis and demonstrating wool fiber cortical fractions.
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The document describes a double embedding technique for sectioning hair and wool fibers. The process involves attaching a cleaned fiber bundle to a wire frame with collodion adhesive, soaking it in nitrocellulose, and treating it with chloroform. The hardened bundle is then embedded in a paraffin-beeswax mixture and sectioned at 6-8 μ. For quantitative study, fibers can be stained with orange G or picric acid, and wool fiber cortical fractions can be demonstrated by staining with polychrome methylene blue after oxidation in a specific solution.
