The palette of techniques for cell cycle analysis

The document reviewed various techniques for cell cycle analysis, emphasizing the importance of precise regulation of the cell cycle for genomic fidelity and coordination with cell fate in multicellular organisms. It highlighted the role of altered cell cycle dynamics in pathophysiological processes and the efforts to define the biochemical machineries involved. The review revisited assumptions of conventional population-based measurements and discussed new tools for addressing cell cycle heterogeneity, particularly in the single-cell era. It evaluated the strengths and weaknesses of methods measuring temporal aspects of the cell cycle and discussed emerging techniques for capturing cell cycle speed at single-cell resolution in live animals.