Mechanism of Traditional Chinese Medicine in the Treatment of Ischemic Stroke by Intervening Ferroptosis Pathway Based on Molecular Target Prediction and Bioinformatics

The study investigates the role of traditional Chinese medicine (TCM) in treating ischemic stroke by targeting the ferroptosis pathway. Using bioinformatics and molecular techniques, researchers identified 410 genes related to ischemic stroke, narrowing down to 9 key genes linked to immune and inflammatory responses. They screened 445 TCMs and 1799 small molecule ligands, validating 10,850 active ingredient-target combinations. Notably, ECH1-118705169 and ECH1-Kaempferol-3,7-O-bis-alpha-L-rhamnoside showed stable binding, suggesting potential therapeutic effects. The study highlights the importance of bitter and cold TCMs in heat clearing and detoxification, offering insights into TCM's molecular mechanisms and its potential in developing treatments for ischemic stroke.