The Inhibition Activity of Tannin on the Formation of Mono-Species and Polymicrobial Biofilm by Escherichia Coli, Staphylococcus Aureus, Pseudomonas Aeruginosa, and Candida Albicans

The study investigated the effectiveness of tannin, a polyphenol compound, in inhibiting and degrading biofilms formed by C. albicans, P. aeruginosa, E. coli, S. aureus, and polymicrobial biofilms. Using the microtiter broth method, the study found that 1% tannin significantly inhibited mono-species biofilm formation, with inhibition rates for S. aureus, E. coli, P. aeruginosa, and C. albicans ranging from 35.13% to 79.04% during different biofilm phases. Tannin also demonstrated the ability to degrade and damage the extracellular polymeric substance matrix of polymicrobial biofilms, suggesting its potential as a new antibiofilm agent. The findings were supported by scanning electron microscopy and statistical analysis using SPSS.