A TA/Cu2+ Nanoparticle Enhanced Carboxymethyl Chitosan-Based Hydrogel Dressing with Antioxidant Properties and Promoting Wound Healing

The study introduces a novel hydrogel dressing made from carboxymethyl chitosan and TA/Cu2+ nanoparticles, designed to enhance wound healing through its antioxidant and antimicrobial properties. In vivo experiments on 24 male Sprague-Dawley rats demonstrated that the hydrogel significantly improved wound healing rates, achieving 94.6% compared to 90.5% for CMCS hydrogel and 77.6% for the control group. The hydrogel's antioxidant capacity was evidenced by an 83% reduction in DPPH free radicals, and it supported over 90% cell viability in HUVECs, enhancing cell migration and angiogenesis. The sustained release of copper ions contributed to tissue repair and regeneration, with histological analysis showing well-aligned tissue structures and collagen accumulation. The CMCS-TA/Cu2+ hydrogel is highlighted as a promising material for wound repair due to its mechanical strength, cytocompatibility, and ability to promote rapid healing without visible scars.