How Storage Post Sampling Influences the Stability of Sebum When Used for Mass Spectrometry Metabolomics Analysis?

This study investigates the stability of sebum samples for mass spectrometry metabolomics analysis, focusing on storage temperature and duration. Sebum samples were collected and stored at ambient, -20 °C, and -80 °C for up to four weeks. Analysis using chromatographic mass spectrometry techniques showed no significant differences in sebum stability based on storage conditions, although volatile sampling was affected by batch effects. The findings suggest that sebum samples are robust to storage conditions, making them suitable for remote and at-home sampling for clinical assays, unlike more sensitive liquid samples.