Population And Individual Stem Cell Dynamics In The Olfactory Epithelium
January 2016
in “
eScholarship (California Digital Library)
”
Studysummary This dissertation reports that horizontal basal cells in the olfactory epithelium undergo symmetric divisions balanced by population asymmetry, which supports their renewal and differentiation during tissue homeostasis and regeneration.
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The study investigated the dynamics of horizontal basal cell (HBC) self-renewal and differentiation in the olfactory epithelium (OE) using lineage tracing and single-cell RNA sequencing. It found that symmetric HBC divisions were balanced by population asymmetry, independent of spindle orientation and cell polarity. At steady-state, HBCs with a conditional knockout of p63 differentiated directly into sustentacular cells without dividing and produced neural progenitors that generated neurons. During regeneration, all HBCs were activated and entered the cell cycle before renewing or differentiating. The sustentacular lineage developed before the neuronal lineage with fewer transcriptional changes. These findings provided insights into HBC dynamics and offered a model for studying adult stem cells in other tissues.
