Raman Spectroscopic Analysis of L-Phenylalanine and Hydrolyzed Eggwhite Protein Penetration into Keratin Fibers

    Akio Kuzuhara
    TLDR L-phenylalanine and hydrolyzed eggwhite protein deeply penetrate human hair.
    The study investigated the penetration of L-phenylalanine (Phe) and hydrolyzed eggwhite protein (HEWP) into keratin fibers of human hair using Raman spectroscopy. It was found that hydrophobic amino acids, like Phe, could penetrate virgin white human hair, while hydrophilic amino acids could not. Phe treatment at 50°C for 16 hours increased its content at various depths of the hair, indicating deep penetration. Similarly, HEWP treatment increased disulfide and random coil contents in bleached human hair, showing that HEWP also deeply penetrated the hair fibers.
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