Clinical and Histological Study of Cold Physical Plasma Jet in Treatment of Full Thickness Skin Wounds of Normal and Diabetic Dogs

The study investigated the effects of homemade helium non-equilibrium atmospheric pressure plasma jet therapy on wound healing in diabetic and non-diabetic dogs. Conducted on 24 dogs, divided into diabetic and non-diabetic groups, each with treatment and control subgroups, the therapy significantly accelerated wound healing. Plasma-treated wounds in both diabetic and non-diabetic treatment subgroups showed early and complete closure by day 30, compared to day 35 or incomplete closure in control subgroups. Histological analysis revealed enhanced epithelization, angiogenesis, new hair follicle formation, collagen fiber development, and inflammation control. The therapy also increased fibroblast proliferation and collagen deposition, with significant differences observed between groups and over time (P<0.001 and P<0.05, respectively). The study concluded that plasma jet therapy improved wound healing quality and speed.