Single Injection of Very Mild Dose Botulinum Toxin in the Vastus Lateralis Improves Testicular Spermatogenesis and Sperm Motility in Aging Experimental Mice

The study investigated the effects of a single, mild dose of botulinum toxin (BoNT) injected into the vastus lateralis muscle on testicular function in ageing mice. The results showed that a dose of 1 U/kg bodyweight of BoNT improved spermatogenesis and increased the activity of key antioxidants in the testis. This led to an enhanced total sperm count and improved sperm motility. The findings suggested that a mild intramuscular dose of BoNT could be a potential treatment strategy for managing and preventing male infertility.