Fine Mapping and Identifying the Mutation Gene of Snthr-1 Bao Scant Hair Mouse

The study aimed to identify the mutant gene responsible for the scant hair phenotype in snthr-1Bao mice, which was mapped to the terminal side of chromosome 9. Researchers used F2 mice from intercrossing (C57BL/6J x snthr-1Bao)F1 mice and analyzed polymorphisms in 2 microsatellites, 35 SSRs, and 3 SNPs for fine mapping. Out of over 4,400 F2 mice, 1,100 scant hair mice were selected, and the mutant gene was narrowed down to a 1.367 Mb region on chromosome 9. The primary candidate gene identified was Plcd1, which was disrupted by a 14,883 bp deletion that also affected Vill. This deletion was likely responsible for the abnormal phenotype observed in the snthr-1Bao mice.