Histochemical Observations of Lectin-Binding Sites and Keratin Distribution in Calcifying Epithelioma of Malherbe

The study investigated the histochemical distribution of lectin binding sites and keratin protein in calcifying epithelioma of Malherbe and compared it to normal hair follicles. Using HRP-conjugated lectin and indirect PAP techniques, it was found that basophilic and shadow cells in the tumor resembled matrix and cortex cells in hair follicles. Con A lectin showed the most prominent binding, particularly in tumor giant cells, which also exhibited strong keratin staining, enhanced by amylase treatment. Lectin staining intensity generally decreased in epithelial and stromal tissues after neuraminidase treatment.