Lack of androgen receptor transcriptional activity in human keratinocytes
June 2000
in “
Journal of dermatological science
”
androgen receptor AR human keratinocytes dihydrotestosterone DHT transient transfection assays chloramphenicol acetyltransferase assays CAT assays AR co-activators ARA70 ARA55 Western blotting exogenous AR AR transcriptional activity androgen receptor AR keratinocytes DHT CAT assays AR co-activators Western blotting
TLDR Human keratinocytes do not naturally respond to androgens.
The study investigated whether human keratinocytes could act as androgen target cells by examining androgen receptor (AR) transcriptional activity. Using transient transfection assays and chloramphenicol acetyltransferase (CAT) assays, it was found that dihydrotestosterone (DHT) induced a very low level of AR activity in HaCaT keratinocytes, which was not enhanced by AR co-activators ARA70 or ARA55. Western blotting confirmed that both HaCaT and normal keratinocytes did not express AR protein. However, transfection of exogenous AR into HaCaT cells restored AR transcriptional activity, indicating that these cells possess the necessary accessory factors for AR activity. The conclusion was that keratinocytes are unlikely to be target cells for androgens.
