Itraq-Based Proteome Profiling Revealed The Role Of Phytochrome A In Regulating Primary Metabolism In Tomato Seedling

The study used iTRAQ-based quantitative proteomics and metabolomics to explore the role of Phytochrome A in regulating primary metabolism in tomato seedlings under far-red light (FR). It was found that Phytochrome A played a crucial role in the FR-mediated development of the mature seedling proteome. In the FR-light insensitive mutant (fri), which has a mutation in Phytochrome A, there was a decreased abundance of enzymes involved in photosynthesis and carbon fixation. Additionally, there was a significant accumulation of storage proteins, suggesting a shift towards converting sugars into storage materials. The increased levels of organic sugars in the mutant seedlings indicated a lack of photomorphogenesis.