Impacts of Iron on Phosphate Starvation-Induced Root Hair Growth in Arabidopsis

    September 2022 in “ Plant Cell & Environment
    Caiwen Xue, Wenfeng Li, Ren Fang Shen, Ping Lan
    TLDR Iron affects root hair growth in Arabidopsis during phosphate deficiency by influencing auxin signaling and vesicle trafficking.
    The study investigated the role of iron (Fe) in phosphate starvation-induced root hair growth (RHG) in Arabidopsis. It was found that Fe influenced RHG at both phosphate sufficient and deficient levels through the generation of reactive oxygen species. The research highlighted that auxin and the central regulator PHR1 were necessary for Fe-triggered RHG under phosphate deficiency. Proteomic analysis showed that Fe affected vesicle trafficking and auxin synthesis and transport, with further validation using a vesicle trafficking inhibitor and an auxin reporter. Specifically, Fe impacted the recycling of PIN2, an auxin efflux transporter, under phosphate deficiency, and root hairs of the pin2 mutant showed reduced responses to Fe. The study proposed that Fe modulated auxin signaling by affecting vesicle trafficking, particularly PIN2 recycling, in conjunction with PHR1 to regulate phosphate starvation-induced RHG.
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