Intravital longitudinal wide-area imaging of dynamic bone marrow engraftment and multilineage differentiation through nuclear-cytoplasmic labeling

This study utilized a H2B-GFP/β-actin-DsRed double-transgenic mouse model to achieve subcellular-level nuclear-cytoplasmic visualization of transplanted bone marrow cells, allowing for dynamic in vivo monitoring of these cells' distribution and proliferation post-bone marrow transplantation (BMT). The research focused on the calvarial bone marrow, using fluorescently labeled vasculature as landmarks to repeatedly identify the same areas. The findings revealed various dynamic behaviors of transplanted bone marrow cells, including cluster formation, migration, and active proliferation in the early stages after transplantation.