In Vitro Growth and Differentiation of Epithelial Cells Derived from Post-Embryonic Hair Follicles

January 1982 in “ Australian Journal of Biological Sciences ”

Dean R. Hewish, Robert C. Marshall

epithelial cells hair follicles mesenchymal cells fibroblast citrulline hair cortical keratin

TLDR Epithelial cells from young rat hair follicles grow and form aggregates in culture, but don't produce hair keratin proteins.

The study investigated the in vitro growth and differentiation of epithelial cells derived from post-embryonic hair follicles of young rats. During the first 2 days of culture, cell aggregates formed more rapidly at higher cell densities, predominantly consisting of epithelial cells with some mesenchymal (fibroblast) cells. Citrulline was detected in the cultured cell proteins. Electrophoretic analysis revealed the presence of hair cortical keratin, although these proteins were not synthesized during the culture period, which contradicted previous findings.

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