An Improved Method for the Histological Preparation of Single Hairs and Dust Samples: Morphological and Immunological Examination

The document reported an improved method for the serological and morphological investigation of human hair. The technique involved fixing hair onto a microscopic slide with cellophane tape to observe the cuticula and medulla. A section of hair containing the medulla was dissected, embedded in paraffin, and cross-sectioned. Immunohistochemistry, specifically the biotin-antibiotin ABC technique, was used to definitively confirm the blood type of the hair. This method could also be applied to dust samples containing shaved beard hair.