Immunohistochemical Demonstration of Breast-Derived and/or Carcinoma-Associated Glycoproteins in Normal Skin Appendages and Their Tumors

The study investigated the expression and localization of specific glycoproteins in 66 benign and malignant skin appendage tumors using monoclonal antibodies GCDFP-15, GU18, B72.3, and VU-1D9. These antibodies were used to stain formalin-fixed, paraffin-embedded tissue, revealing different localization patterns in normal eccrine and apocrine sweat glands. Notably, B72.3 did not stain eccrine glands or eccrine-derived tumors. GUI8 and VU-1D9 were effective in labeling mature sebocytes and sebaceous carcinomas, while VU-1D9 also marked secondary germ cells in hair follicle bulbs and basaloid cells in trichoepitheliomas and basal cell carcinomas. The study concluded that these monoclonal antibodies were valuable markers for studying normal skin appendages and their tumors.