The Heme-Responsive PrrH sRNA Regulates Pseudomonas Aeruginosa Pyochelin Gene Expression

This study investigates the role of the heme-responsive PrrH sRNA in Pseudomonas aeruginosa, particularly its regulation of pyochelin gene expression. The researchers conducted transcriptomic and proteomic analyses, revealing that pyochelin synthesis proteins increased in prrH mutants compared to wild type, despite unchanged mRNA levels. They found potential direct regulation by PrrH through complementarity with the pchE mRNA sequence. The study also noted that pchE mRNA levels rose in prrH mutants under static conditions, emphasizing the importance of environmental factors like light in PrrH's regulatory function. This research identifies pyochelin biosynthesis genes as a novel target of PrrH, linking heme availability to siderophore biosynthesis in P. aeruginosa.