Genotoxicity of Rice Bran Oil Extracted by Supercritical CO2 Extraction

Rice bran oil extracted by supercritical CO2 (RB-SCE) was investigated for its potential genotoxic effects using three short-term mutagenicity assays. The study found that RB-SCE exhibited no genotoxicity in the bacterial reverse mutation assay up to 5000 mg/plate and in the in vivo micronucleus test up to 600 mg/kg body weight. However, RB-SCE showed significant genotoxicity in the in vitro chromosome aberration test at 120 µg/mL with S9 mix and 200 µg/mL without S9 mix. Despite this, the induction of chromosomal aberrations was considered to have no biological significance, leading to the conclusion that RB-SCE can be regarded as a non-genotoxic material based on the available in vivo and in vitro results.