Capillary Gas-Chromatographic Determination of Spermidine in Hair Lotion

    May 2006 in “ Journal of Separation Science ”
    Veniero Gambaro, Eleonora Casagni, Lucia Dell’Acqua, Mauro Valenti, Giacomo Luca Visconti
    TLDR The method effectively measures spermidine in hair lotions.
    The study developed a capillary gas-chromatographic method to quantitatively determine spermidine (SPD) in hair lotions, which was believed to promote hair growth. The method involved using 1,6-diaminohexane as an internal standard, a methyl silicone capillary column, and a flame ionisation detector. Hair lotion samples were treated with an alkaline solution, extracted with diethyl ether containing ethyl chloroformate, and analyzed after reconstitution in ethyl acetate. The method was validated for specificity, linearity, precision, and accuracy, confirming its effectiveness for measuring SPD in hair lotions.
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