Capillary Gas-Chromatographic Determination of Spermidine in Hair Lotion

May 2006 in “ Journal of Separation Science ”

Veniero Gambaro, Eleonora Casagni, Lucia Dell’Acqua, Mauro Valenti, Giacomo Luca Visconti

The study developed a capillary gas-chromatographic method to quantitatively determine spermidine (SPD) in hair lotions, which was believed to promote hair growth. The method involved using 1,6-diaminohexane as an internal standard, a methyl silicone capillary column, and a flame ionisation detector. Hair lotion samples were treated with an alkaline solution, extracted with diethyl ether containing ethyl chloroformate, and analyzed after reconstitution in ethyl acetate. The method was validated for specificity, linearity, precision, and accuracy, confirming its effectiveness for measuring SPD in hair lotions.

View this study on analyticalsciencejournals.onlinelibrary.wiley.com →

Discuss this study in the Community →

Research cited in this study

1 / 1 results

research Inhibition of Polyamine Synthesis Alters Hair Follicle Function and Fiber Composition

42 citations, February 1996 in “Journal of Investigative Dermatology”

The study investigated the role of polyamines in hair follicle function and fiber composition in sheep. It found that inhibiting ornithine decarboxylase with alpha difluoromethylornithine altered fiber characteristics but did not inhibit wool follicle growth in culture. Conversely, inhibiting S-adenosylmethionine decarboxylase with methylglyoxal (bis)guanylhydrazone completely inhibited fiber growth, which could be reversed by adding spermidine, but not spermine. The study concluded that polyamines, particularly spermidine, were crucial for hair growth, as evidenced by the ability of spermidine to partially counteract growth depression in methionine-deficient conditions.