Expression of Bioactive Recombinant Human Fibroblast Growth Factor 10 in Carthamus Tinctorius L. Seeds
October 2017
in “
Protein expression and purification
”
recombinant human fibroblast growth factor 10 rhFGF10 Carthamus tinctorius safflower oilbody-oleosin technology Agrobacterium tumefaciens transformation oleosin-rhFGF10 MTT assays cellular proliferation hair growth tissue repair burn wounds FGF10 Agrobacterium transformation cell growth wound healing
TLDR Scientists made safflower seeds produce a human growth factor that could help with hair growth and wound healing.
In the study from October 2017, researchers successfully expressed recombinant human fibroblast growth factor 10 (rhFGF10) in safflower (Carthamus tinctorius L.) seeds using oilbody-oleosin technology. They constructed a plant expression vector and introduced it into safflower plants through Agrobacterium tumefaciens transformation. The transformed plants were then grafted to obtain mature plants. The expression of oleosin-rhFGF10 was confirmed in the safflower seeds and was found to be heritable up to the T3 generation. Additionally, MTT assays indicated that the oil bodies expressing oleosin-FGF10 promoted cellular proliferation in a dose-dependent manner. This research suggests a potential method for producing rhFGF10 to meet its growing demand in pharmacology, particularly for applications in hair growth, tissue repair, and treatment of burn wounds.
