Effects of storage culture media, temperature and duration on human adipose‑derived stem cell viability for clinical use

The study investigated the optimal conditions for short-term storage of human adipose-derived stem cells (ADSCs) to maintain their viability for clinical use. It found that storing ADSCs at 4°C in 10% human serum (HS) was the most effective method, providing the highest survival rate and avoiding the need for cryoprotectants, which can cause toxicity. This method outperformed storage in 10% platelet-rich plasma (PRP) and 0.9% NaCl. The study emphasized that ADSCs should be used within 2 to 4 hours of storage to ensure their functionality, highlighting the importance of standardizing ADSC preparation and storage for clinical applications.