Effect of Liquid Blood Concentrates on Cell Proliferation and Cell Cycle- and Apoptosis-Related Gene Expressions in Nonmelanoma Skin Cancer Cells: A Comparative In Vitro Study

This study explores the effects of platelet-rich fibrin (PRF) on nonmelanoma skin cancer cells, specifically basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). PRF treatment significantly reduced cell viability and proliferation, particularly in BCC cells, by inducing cell cycle arrest and activating apoptotic pathways. This was evidenced by the downregulation of CCND1 and upregulation of p21, p53, caspase-8, and caspase-9 gene expressions. The study suggests that PRF may have tumor-suppressive effects, offering a promising basis for future research into its use in managing localized skin cancers.