Development of a FRET-based 3D in vitro breast tumor model and its application in evaluating drug-induced apoptosis
January 2017
TLDR A new 3D breast tumor model helps test drug effects more accurately than traditional methods.
The study developed a novel FRET-based 3D in vitro breast tumor model to evaluate drug-induced apoptosis in real-time, addressing limitations of traditional 2D cultures. The model used a FRET-based C3 biosensor to detect caspase-3 activation, providing non-invasive, longitudinal analysis of drug responses. When tested with paclitaxel, the model showed a dose- and time-dependent reduction in FRET effect, demonstrating its potential for drug screening. Additionally, the study incorporated fibroblasts into the 3D model, creating a co-culture system that more closely resembled in vivo tumors, enhancing the accuracy of drug response evaluations. The research highlighted the model's utility in bridging the gap between 2D and animal models for efficient drug efficacy analysis.
