Conditioned medium from primary cytotrophoblasts, primary placenta-derived mesenchymal stem cells, or sub-cultured placental tissue promoted HUVECs angiogenesis in vitro

The study demonstrated that conditioned medium (CM) from primary cytotrophoblasts (CTBs), human placenta-derived mesenchymal stem cells (hPDMSCs), and sub-cultured placental tissue promoted angiogenesis in human umbilical vein endothelial cells (HUVECs) in vitro. The research found that CM from early pregnancy placental cells had a more significant effect on angiogenesis than those from full-term pregnancy. CM collected at specific time points (24-48 hours for placental cells and 7 days for sub-cultured tissue) showed the best results. The study identified 18 angiogenic factors in the CM, with CXCL-5, GRO, IL-6, IL-8, and MCP-1 being the most prominent in sub-cultured tissue-derived CM. These findings provided insights into placental angiogenesis regulation and suggested potential clinical applications for placental components in vascular tissue engineering.