Conditioned medium from primary cytotrophoblasts, primary placenta-derived mesenchymal stem cells, or sub-cultured placental tissue promoted HUVEC angiogenesis in vitro

The study demonstrated that conditioned medium (CM) from primary cytotrophoblasts, placenta-derived mesenchymal stem cells (hPDMSCs), and sub-cultured placental tissue promoted angiogenesis in human umbilical vein endothelial cells (HUVECs) in vitro. CM from early-pregnancy placental cells had a more significant impact on angiogenesis than those from full-term pregnancy, with the most substantial effects observed when CM was collected at specific time points (24-48 hours for placental cells and 7 days for sub-cultured tissue). The study identified 18 angiogenic factors in the CM, with CXCL-5, GRO, IL-6, IL-8, and MCP-1 being prominent in sub-cultured placental tissue-derived CM. These findings suggested potential clinical applications for placental components in vascular tissue engineering and treatments, although further in vivo studies were needed to confirm these results.