Conditioned Medium From Primary Cytotrophoblasts, Primary Placenta-Derived Mesenchymal Stem Cells, Or Placental Tissue Promoted HUVECs Angiogenesis In Vitro

The study investigated the effects of conditioned medium (CM) from primary cytotrophoblasts (CTBs), primary human placenta-derived mesenchymal stem cells (hPDMSCs), and placental tissue on angiogenesis in human umbilical vein endothelial cells (HUVECs) in vitro. It was found that CM from both placental cells and tissue promoted angiogenesis by enhancing proliferation, adhesion, migration, invasion, and tube formation of HUVECs. Early pregnancy placental cells had a more significant impact than those from later stages, and CM from placental tissue was more effective than CM from individual cell types. The study identified 18 angiogenic factors in placental tissue-derived CM, with CXCL-5, GRO, IL-6, IL-8, and MCP-1 being the most prominent. This research provided insights into placental angiogenesis regulation and suggested potential clinical applications for placental components in vascular tissue engineering and treatments.