Molecular Cloning of 5′ Flanking Region of Ovine Keratin Associated Protein 6-1 Gene and Sequence Comparison

The study involved designing and synthesizing specific oligonucleotide primers for the ovine keratin associated protein 6-1 gene, amplifying a 1042 base pair fragment using polymerase chain reaction, and inserting it into the EcoRV site of the pMD19-T vector. The recombinant clones were screened and sequenced, showing high identity with the original sequence. This work laid the groundwork for creating transgenic animals and exploring the mechanisms of hair regulatory genes expressed in hair follicle cells.