Bioactivity Guided Isolation and Characterization of the Phytoconstituents from Tridax Procumbens

In the 2011 study, researchers explored the immunomodulatory effects of Tridax procumbens L., a plant traditionally used in Ayurvedic medicine. They created fractions from a methanol extract of the plant, specifically chloroform, ethyl acetate, and n-butanol fractions, and tested them on mice. The ethyl acetate and n-butanol fractions exhibited significant immunomodulatory activity, with the ethyl acetate fraction containing the highest amount of phenolic content. Three phytochemicals, named SA-3, SA-4, and SA-6, were isolated, with SA-4 and SA-6 showing a dose-dependent enhancement of immune response in mice at doses of 2 and 4 mg/kg over fifteen days. The chemical structures of these compounds were identified using various spectroscopic methods. The study concluded that the identified compounds have potential as immunostimulators and highlighted the importance of further clinical trials in immunocompromised patients.