A Partially Automated Method for DNA Extraction From Marmoset Hair Follicles to Avoid Blood Chimerism

September 2025 in “ OPAL (Open@LaTrobe) (La Trobe University) ”

Alexandra M. Stendahl (22313950), Qiangge Zhang (263901), Ana C. Lima (22313953), Curtis Mello (22313956), James Nemesh (84599), Sam Peterson (3474194), Jenna Castro (22313959), Fritzie T. Celino-Brady (22313962), Karina Ray (4397881), Xian Gao (5498990), Yuanyuan Hou (130870), Chenjie Shen (22313965), Katinka A. Vigh-Conrad (22313968), Fenna Krienen (22313971), Guoping Feng (13275), Steven A. McCarroll (22313974), Donald F. Conrad (9221365), Ricardo C. H. del Rosario (22313977)

DNA extraction hair follicles proteinase K Promega Maxwell RSC system

TLDR Researchers created an efficient method to extract DNA from marmoset hair, reducing blood chimerism.

Researchers developed a non-invasive, partially automated method for extracting DNA from marmoset hair follicles to address the issue of blood chimerism in these primates. This protocol, which uses proteinase K and Promega’s Maxwell RSC system, was tested on samples from over 300 animals across two species. It successfully produced high-quality whole genome sequencing libraries from approximately 150 hair follicles, with low chimerism rates of 2.3% and an average duplication rate of 0.19. The method is efficient, scalable, and suitable for projects requiring low-input DNA sources or high-throughput processing. Data from the study are available in supplementary tables and archived online.

Discuss this study in the Community →

Similar Research

5 / 1000+ results

research A Partially Automated Method for DNA Extraction from Marmoset Hair Follicles to Avoid Blood Chimerism

September 2025 in “OPAL (Open@LaTrobe) (La Trobe University)”

Researchers created an efficient method to extract DNA from marmoset hair, avoiding blood chimerism.

research A Partially Automated Method for DNA Extraction from Marmoset Hair Follicles to Avoid Blood Chimerism

September 2025 in “Frontiers in Genetics”

The method effectively extracts high-quality DNA from marmoset hair, avoiding blood chimerism.

research Beta-Caryophyllene Enhances Wound Healing Through Multiple Routes

67 citations , December 2019 in “PloS one”

Beta-caryophyllene helps improve wound healing in mice, especially in females.

research A Monoallelic Two-Hit Mechanism in PLCD1 Explains the Genetic Pathogenesis of Hereditary Trichilemmal Cyst Formation

21 citations , October 2019 in “The journal of investigative dermatology/Journal of investigative dermatology”

A specific gene change plus an additional mutation in the same gene cause hereditary trichilemmal cysts.

research Beta-Caryophyllene Enhances Wound Healing Through Multiple Routes

3 citations , April 2019 in “bioRxiv (Cold Spring Harbor Laboratory)”

Beta-caryophyllene, found in essential oils, helps wounds heal better in multiple ways.

A Partially Automated Method for DNA Extraction From Marmoset Hair Follicles to Avoid Blood Chimerism

Related Community Posts Join

community So we have 20k dollars human like machines available but no new effective treatment for hairloss since finasteride :)

community Possible natural pp405 solution

community Looks like a real cure to the root of baldness (DHT-induced senescence) was proven earlier this year

community People like this don't have to be bald.

community Baldness Breakthrough: microRNA Stimulates Hair Growth in Aging Follicles

community 10 Days Post-Op: Hair Transplant Results and Experience

Similar Research

research A Partially Automated Method for DNA Extraction from Marmoset Hair Follicles to Avoid Blood Chimerism

research A Partially Automated Method for DNA Extraction from Marmoset Hair Follicles to Avoid Blood Chimerism

research Beta-Caryophyllene Enhances Wound Healing Through Multiple Routes

research A Monoallelic Two-Hit Mechanism in PLCD1 Explains the Genetic Pathogenesis of Hereditary Trichilemmal Cyst Formation

research Beta-Caryophyllene Enhances Wound Healing Through Multiple Routes