A Partially Automated Method for DNA Extraction From Marmoset Hair Follicles to Avoid Blood Chimerism

    September 2025 in “ OPAL (Open@LaTrobe) (La Trobe University) ”
    Alexandra M. Stendahl (22313950), Qiangge Zhang (263901), Ana C. Lima (22313953), Curtis Mello (22313956), James Nemesh (84599), Sam Peterson (3474194), Jenna Castro (22313959), Fritzie T. Celino-Brady (22313962), Karina Ray (4397881), Xian Gao (5498990), Yuanyuan Hou (130870), Chenjie Shen (22313965), Katinka A. Vigh-Conrad (22313968), Fenna Krienen (22313971), Guoping Feng (13275), Steven A. McCarroll (22313974), Donald F. Conrad (9221365), Ricardo C. H. del Rosario (22313977)
    Studysummary This study developed a partially automated protocol utilizing hair follicles for DNA extraction in marmosets, achieving reliable whole genome sequencing with low chimerism, offering an efficient alternative to blood for genetic studies in non-human primates.
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    Researchers developed a non-invasive, partially automated method for extracting DNA from marmoset hair follicles to address the issue of blood chimerism in these primates. This protocol, which uses proteinase K and Promega’s Maxwell RSC system, was tested on samples from over 300 animals across two species. It successfully produced high-quality whole genome sequencing libraries from approximately 150 hair follicles, with low chimerism rates of 2.3% and an average duplication rate of 0.19. The method is efficient, scalable, and suitable for projects requiring low-input DNA sources or high-throughput processing. Data from the study are available in supplementary tables and archived online.
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