Angiogenic Effects of Recombinant Thymosin Beta 4 in a Mouse Hindlimb Ischemia Model

The study examined the effects of recombinant thymosin β4 (rTβ4) on angiogenesis in a mouse model of hindlimb ischemia, involving 16 mice treated with rTβ4 and 6 with saline. Results indicated that rTβ4 increased the expression of angiogenic markers HIF-1α, VEGF-1, and eNOS, and reduced muscle fibrosis, as evidenced by histopathological analysis. Immunohistochemistry showed higher CD31 expression, indicating enhanced microvascular formation. Overall, rTβ4 was found to promote angiogenesis and reduce muscle fibrosis, suggesting its potential as a therapeutic option for peripheral arterial disease.