Characterization of Aberrant mTOR Signaling Pathway in U87MG Glioblastoma Cell Line by Quantitative Phosphoproteomics
January 2021
TLDR Inhibiting mTORC2 can reduce DNA repair and increase cancer cell death, suggesting potential for targeted brain cancer treatments.
The study investigated the aberrant mTORC2 signaling pathway in U87MG glioblastoma cells using quantitative phosphoproteomics. Researchers used inhibitors AZD8055 and rapamycin to analyze the effects on phosphorylated peptides, identifying numerous mTORC2 downstream targets. They found that mTORC2 inhibition affected DNA damage repair, particularly through the phosphorylation of BABAM1 at Ser29, which is crucial for DNA repair and cancer cell survival. Inhibiting mTORC2 reduced DNA repair activities and promoted apoptosis. The study suggested that mTORC2 plays a significant role in oncogenic DNA damage response, potentially guiding future brain cancer treatments.
