[A three-dimensional observation of human hair tissue treated by modified trypsin-HC1 method].

The study utilized a modified trypsin-HC1 method combined with ultrasonic cleaning to observe the three-dimensional ultrastructures of human hair and hair follicles under a scanning electron microscope. This treatment effectively removed connective tissues, allowing detailed observation. Key findings included the identification of a basal plate composed of fibroblasts and collagen fibers, a tightly surrounding fibrous connective tissue sheet with a capillary network around the lower hair follicle, and morphological differences in cell layers of the hair bulb. Additionally, numerous micro-villous projections were observed on the outer root sheath cells and keratinized inner root sheath, with the latter resembling tree bark. This method proved useful for detailed ultrastructural observation of hair tissues.