A Three-Dimensional Observation of Human Hair Tissue Treated by Modified Trypsin-HCl Method

The study conducted by H Murai and colleagues in 1989 focused on observing the three-dimensional ultrastructures of human hair and hair follicles using a modified trypsin-HC1 method combined with ultrasonic cleaning. This method effectively removed connective tissues surrounding the hair follicle, allowing detailed observation under a scanning electron microscope. Key findings included the identification of a basal plate composed of fibroblasts and collagen fibers, a tightly surrounding fibrous connective tissue sheet with a capillary network, and morphological differences in cell layers of the hair bulb. Additionally, numerous micro-villous projections were noted on the surfaces of outer root sheath cells and keratinized inner root sheath, with the latter resembling tree bark. This modified method was deemed useful for detailed three-dimensional ultrastructural studies of human hair and follicles.