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GlossaryIndirect Immunofluorescence (Indirect Fluorescent Antibody Technique)

technique using fluorescent-tagged antibodies to detect specific proteins

Indirect Immunofluorescence (IIF) is a laboratory technique used to detect specific antibodies or antigens in a sample. It involves applying a primary antibody to the target antigen, followed by a secondary antibody that is tagged with a fluorescent dye. When viewed under a fluorescence microscope, the areas where the antibodies have bound will glow, allowing researchers to visualize the presence and location of the target molecules. This method is widely used in medical diagnostics and research, including the study of autoimmune diseases and infections.

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community Pyrilutamide ,the molecule’s true nature

in Research 302 upvotes 3 months ago

Pyrilutamide is a selective AR antagonist with a high binding affinity, making it effective in competing with DHT for androgen receptors. The 1% concentration is more effective than the 0.5%, but the latter may suffice for mild hair loss; the drug is considered a good option for those avoiding 5AR blockers due to side effects.

community So it turns out Post Finasteride Syndrome was never real?

in Finasteride/Dutasteride 271 upvotes 3 months ago

Post Finasteride Syndrome (PFS) is debated, with some users reporting severe side effects from finasteride, while others believe these effects are rare or psychosomatic. Treatments discussed include finasteride, minoxidil, and RU58841.

community Shampoo except ketoconazole that works

in Product 7 upvotes 6 months ago

The conversation is about seeking shampoos that help with hair loss or hair thickening without ketoconazole, suggesting alternatives like minoxidil, finasteride, or stemoxydine. One reply suggests that most hair loss shampoos don't work as claimed.

community 2 months on topical Finasteride, Minoxidil.

in Progress Pictures 37 upvotes 9 months ago

The user started using 0.05% topical finasteride and minoxidil for hair loss treatment on December 15, also incorporating dermarolling and rosemary oil, and is satisfied with the visible improvement in their receding hairline. Another user inquired about where to obtain topical finasteride.

community Full Album of My Results - Dermaroller/pen + minox

in Progress Pictures 586 upvotes 2 years ago

User 74775446 shares successful hair regrowth results using dermaroller/pen and minoxidil. Others discuss their experiences and concerns about finasteride side effects.

community Creatine made all my high DHT symptoms return

in Research/Science 78 upvotes 2 weeks ago

Creatine may increase DHT levels, potentially causing symptoms like acne, oily hair, and hair shedding. Users report mixed experiences, with some avoiding creatine due to hair loss concerns and others seeing no impact.

community Solving Hair Loss with Research at MIT

in Research/Science 590 upvotes 1 month ago

Hair loss treatments like minoxidil, finasteride, TDM-105795, and PP405. MIT is hosting a hackathon to find innovative hair loss solutions.

community Any finasteride users have a history of depression?

in Finasteride/Dutasteride 26 upvotes 2 months ago

The conversation discusses finasteride for hair loss, especially among those with a history of depression. Users shared mixed experiences, with some reporting no issues and others experiencing worsened depression.

community Shedding again after 6 months of great results.

in Minoxidil 15 upvotes 3 months ago

A user experienced shedding after 7 months on Fin/Min spray despite good results and no change in treatment. Other users noted similar experiences and mentioned that shedding periods can be normal.

community Whoever had success with PYRILUTAMIDE, please give us hope outside of 5AR inhibitors.

in Product 7 upvotes 3 months ago

Users discuss using pyrilutamide for hair loss, seeking alternatives to 5AR inhibitors. They mention using minoxidil, ketoconazole shampoo, and RU58841.

community Why is nobody studying the difference between donor and balding hair?

in Chat 89 upvotes 5 months ago

The conversation discusses why the difference between donor and balding hair isn't studied more to find a cure for hair loss. Treatments mentioned include Minoxidil, finasteride, and RU58841.

community Post Finasteride Syndrome, A dangerous lie.

in Chat 142 upvotes 9 months ago

A user initially had side effects from finasteride, attributed them to anxiety and the nocebo effect, and after resuming the drug, experienced positive effects and now warns against misinformation about Post Finasteride Syndrome.

community Study of thousands of Fin users over 20 years finds no evidence of Fin induced sexual dysfunction

in Research/Science 371 upvotes 9 months ago

The conversation is about a finding that finasteride does not significantly increase the risk of sexual dysfunction, with users sharing mixed personal experiences regarding side effects.

community Testosterone in Reference Range Doesn't Cause Hair Loss

in Research/Science 51 upvotes 9 months ago

Testosterone within the normal range does not significantly contribute to male pattern baldness (MPB); DHT is the main factor that can be controlled. Genetics play a crucial role in hair loss, and treatments like finasteride and dutasteride, which block DHT, can help despite potentially raising testosterone levels.

community Yes, most models are absolutely on finasteride

in Finasteride/Dutasteride 1104 upvotes 9 months ago

The conversation discusses how many male models and celebrities likely use finasteride to prevent hair loss, with some expressing surprise that it's not just good genetics keeping their hair intact. Specific treatments mentioned include finasteride and, in one query, minoxidil.

community Does whey protein and creatine aggravate hair loss?

in Research/Science 82 upvotes 11 months ago

The conversation discusses whether whey protein and creatine worsen hair loss. Some users report hair loss with creatine use, while others do not; opinions on whey protein's effects are mixed. Treatments mentioned include PRP, minoxidil, and finasteride.

community Got my bloodwork done today, I have been on fin for 7+ months. 21m

in Update 32 upvotes 1 year ago

A user on finasteride for 7+ months shared bloodwork results, questioning if high DHT levels and testosterone could affect cholesterol. Replies suggest consulting a doctor and question the high testosterone levels.

community Creatine and Finasteride together - still started losing hair

in Finasteride/Dutasteride 20 upvotes 1 year ago

User experienced hair loss after taking creatine with finasteride for 8 years. Others shared similar experiences, but some suggested it could be a minoxidil shed.

community HLT Megathread on HMI-115 (key takeaways in comments)

in Research/Science 40 upvotes 1 year ago

HMI-115, a newly discovered hair loss treatment that could potentially be effective for those with diffuse thinning and telogen effluvium. It is based on prolactin receptor antagonist signaling and has already undergone Phase I trials in women, with potential commercialization by 2027.

community Why I believe PFS is not a "real" condition (and what I believe it is instead)

in Finasteride/Dutasteride 14 upvotes 1 year ago

The post argues that Post-Finasteride Syndrome (PFS) is likely not real and suggests symptoms may be due to mental health issues or the nocebo effect. The conversation includes personal experiences with finasteride, highlighting both positive and negative effects, and emphasizes consulting doctors and using reliable sources for medication.

community Scalp tension and thickness of the galea aponeurotica, inflammation and potential treatment pathways

in Research/Science 2 years ago

Scalp tension potentially affecting hair loss, and potential treatments for male pattern baldness such as Minoxidil, Finasteride and RU58841. Evidence from a study was discussed which suggests that the cause of MPB lies within the follicle itself and is not dependent on its surrounding environment.

community Fluridil/Topilutamide (Eucapil) gave me achy balls, but seems to work

in Treatment 9 upvotes 4 years ago

Treatments for hair loss, focusing on Fluridil/Topilutamide (Eucapil), Finasteride and RU58841 (Alfatradiol). The user reported that the Fluridil seemed to work well but had side effects of testicle ache, decreased mood and sex drive.

community CASSIOPEA ANNOUNCES VERY POSITIVE PHASE II TWELVE MONTHS RESULTS FOR BREEZULA® (CLASCOTERONE) IN TREATING ANDROGENETIC ALOPECIA

in Update 162 upvotes 5 years ago

Clascoterone (Breezula) showed positive results for treating hair loss without affecting cortisol levels. Opinions vary, with some users optimistic about its potential and others critical of its effectiveness compared to existing treatments like finasteride.

community Having androgen receptor density and sensitivity at wrong f*ing place

in Satire 7 upvotes 1 month ago

The conversation discusses hair loss treatments, focusing on the use of topical minoxidil, microneedling, finasteride, and dutasteride, while debating the role of testosterone and DHT in hair loss. It also touches on the potential liver health impacts of these treatments and the genetic sensitivity of hair follicles to androgens.

community Haircafe on why microneedling is bad for hair growth

in Microneedling 157 upvotes 3 months ago

Microneedling combined with minoxidil and finasteride shows significant hair regrowth, though opinions on its effectiveness vary. Some users report substantial benefits, while others highlight the need for more research and consistency in application.

community 7 month progress - Thank you to this community!

in Progress Pictures 409 upvotes 1 year ago

A 21-year-old used 1.25mg finasteride and minoxidil twice daily for hair regrowth, reducing finasteride to 5 times a week since September. He also started using Nizoral for dandruff, noticed an increased appetite, and experienced a temporary change in sex drive.

community M19 shed after 1yr on dutasteride

in Finasteride/Dutasteride 23 upvotes 1 month ago

A 19-year-old male experienced hair shedding after one year on dutasteride and is considering adding topical minoxidil despite scalp itching. Users suggest continuing dutasteride, maintaining a consistent hair routine, and possibly increasing minoxidil dosage.

community It's amazing how much younger you look with intact hair. Picture on the right was taken 3 years after picture on the left. I used the regular finasteride, minoxidil, nizoral combo.

in Progress Pictures 171 upvotes 1 year ago

User regrew hair using finasteride, minoxidil, and nizoral. Another user saw results with dutasteride, minoxidil, and microneedling.

community High Nicotine use and Hair Loss

in Chat 79 upvotes 3 months ago

Heavy nicotine use can lead to hair thinning and loss, but quitting can reverse these effects. The user noticed significant hair regrowth after quitting vaping.

community Wish me luck,i hope these will help ne regrow my hair

in Minoxidil 8 upvotes 7 months ago

A user hopes to regrow hair using a routine of Min/fin solution twice daily, dermarolling twice weekly, multivitamins daily, and biotin twice daily. Another user doubts the effectiveness of biotin and multivitamins.