Establishment of Tsc2-Deficient Rat Embryonic Stem Cells

    March 2015 in “ International Journal of Oncology ”
    YOSHITAKA ITO, Haruna Kawano, Fumio Kanai, Eri Nakamura, Norihiro Tada, Setsuo Takai, Shigeo Horie, Hajime Arai, Toshiyuki Kobayashi, Okio Hino
    TLDR Tsc2-deficient stem cells can help understand and treat TSC-related tumors.
    The study focused on Tuberous sclerosis complex (TSC), an autosomal dominant disorder caused by TSC1 or TSC2 mutations, which leads to tumor development in various organs. Researchers derived embryonic stem cells (ESCs) from Eker rats with the Tsc2 mutation to understand the molecular basis of the disease. They successfully established Tsc2+/+, Tsc2+/-, and Tsc2-/- ESCs, confirming their pluripotency and ability to differentiate into all three germ layers. Gene expression analysis showed that Tsc2-/- ESCs had a distinct profile compared to Tsc2+/+ and Tsc2+/- ESCs. The study suggested that in vitro and in vivo differentiation experiments using these ESCs could elucidate the tissue-specific pathogenesis of the Tsc2 mutation and help identify new therapeutic targets.
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