Transcriptome Analysis Methods: From the Serial Analysis of Gene Expression and Microarray to Sequencing New Generation Methods
December 2023
in “
Biointerface Research in Applied Chemistry
”
TLDR RNA-Seq is now the standard for detailed gene expression analysis.
The document discusses the evolution of transcriptome analysis methods, highlighting the transition from microarray and serial analysis of gene expression to next-generation sequencing (RNA-Seq). RNA-Seq has become a standard method due to its ability to provide comprehensive gene expression analysis and uncover novel RNA species, surpassing the limitations of microarray technology. The study emphasizes the impact of next-generation sequencing on transcriptome analysis and its potential to explore RNA biogenesis and metabolism through advanced cDNA library preparation techniques.
