Transcription factor-based transdifferentiation of human embryonic to trophoblast stem cells

    September 2024 in “ Development ”
    Paula A. Balestrini, Ahmed Abdelbaki, Afshan McCarthy, Liani Devito, Claire E. Senner, Alice E. Chen, Prabhakaran Munusamy, Paul Blakeley, Kay Elder, Phil Snell, Leila Christie, Paul Serhal, Rabi Odia, Mahesh Sangrithi, Kathy K. Niakan, Norah M. E. Fogarty
    The study investigates the transdifferentiation of human embryonic stem cells (hESCs) into trophoblast stem cells (iTSCs) using transcription factors GATA2, GATA3, TFAP2C, KLF5, and MYC. Researchers successfully generated iTSCs from hESCs, which exhibit characteristics similar to human trophoblast stem cells (hTSCs), including self-renewal and specific gene expression profiles. The study highlights the potential of transcription factor-based methods to model placental biology and aid in the study of placental-associated diseases. The findings suggest that while a combination of GATA2, GATA3, and MYC can induce iTSCs, additional factors are needed for long-term maintenance, providing insights into cell differentiation and potential therapeutic strategies.
    Discuss this study in the Community →