4 citations
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May 2013 in “Dermatologic Surgery” Three new techniques simplify and improve the preparation of tissue samples for skin cancer surgery.
2 citations
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February 2024 in “STAR Protocols” In this research, an optimized protocol was developed for dissociating human scalp tissue to produce high-quality single-cell suspensions suitable for single-cell RNA sequencing, aimed at studying the transcriptomics of human hair follicles.
28 citations
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June 2003 in “Applied immunohistochemistry & molecular morphology” This study demonstrated that combining cell conditioning with mild protease digestion enhanced the visualization of versican mRNA in formalin-fixed mouse skin tissue sections compared to using either technique alone.
August 2024 in “STAR Protocols” This study details a step-by-step protocol for preparing mammalian skin samples, including hair follicles, for spatial transcriptomics to overcome challenges inherent in applying this technique to the skin.
2 citations
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January 2020 The document describes a method for preparing hair for microscopy by embedding it in plastic, cutting it, and storing it cold before imaging.