The microfibrillar proteins of human hair: Separation by high-performance liquid chromatography and isolation of some proteins enriched in glycine and tyrosine

The study investigated the microfibrillar proteins of human hair using reversed-phase high-performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A new chromatographic method was developed to isolate these proteins from other hair-matrix proteins, separating them into collectable fractions. The proteins were categorized into two major subgroups, further resolved into six components. Notably, the study identified and separated a group of proteins rich in glycine and tyrosine, previously undetected in human hair. Comparative electrophoretic analysis revealed five bands with molecular weights ranging from 47,000 to 62,000, and the relationship between these bands and chromatographic fractions was under further investigation.