TLDR The modified osmium method improves hair cortex staining for better visualization.
The study described a modified staining method for human hair using transmission electron microscopy, which involved en bloc treatment with a chemical reductant followed by osmium tetroxide. This method improved reagent penetration through a cut fibre end, allowing better visualization of the hair cortex's ultrastructural features. The modified method was compared to other staining techniques, such as en bloc silver nitrate and section stains using uranyl acetate and lead citrate, among others. The study also briefly examined the effects of hair-care treatments on staining quality.
18 citations
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December 2008 in “Journal of structural biology” Hair curvature in Japanese people is linked to specific cell types and filament arrangements in the hair cortex.
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November 2010 in “Journal of morphology” Antler velvet hair and body hair of red deer have different structures that help with protection and insulation.
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