STUDIES ON THE STAINING REACTION OF THE FLUORESCENT CONJUGATE OF GLOBULIN

The study investigates the staining reactions of fluorescent globulin on various tissue types, focusing on the differential staining between normal and tumor cells. It was found that normal cells stained well, while tumor cells did not, likely due to the loss of basic proteins in tumor cells. The study also explored the effects of pH, ion concentration, and fixatives on staining, finding that more alkaline solutions improved staining intensity. Different tissues exhibited varying staining affinities, with some tissues showing bright fluorescence and others none. The study suggests that both the dye and protein components of the fluorescent conjugate contribute to the staining reaction, and further investigation is needed to fully understand the mechanisms involved.