Purification and Calcium Dependence of Transglutaminases from Sheep Hair Follicles

    January 1997 in “ Bioscience Biotechnology and Biochemistry ”
    Yoshiyuki Kumazawa, Tomoko Ohtsuka, Daiki Ninomiya, Katsuya Seguro
    The study investigated the calcium dependence of transglutaminases from sheep hair follicles, which were previously thought to be calcium-independent. Two types of transglutaminases, DU-TG and DA-TG, were purified and analyzed. DU-TG had molecular masses of 77 and 82 kDa, while DA-TG had masses of 40 and 80 kDa. Both enzymes were found to be calcium-dependent, with maximum activation at 1 mM CaCl2 for DU-TG and 0.1 mM CaCl2 for DA-TG, and contained cysteine residues in their active sites, similar to other mammalian transglutaminases.
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