PTP1B Inhibitory and Anti-inflammatory Properties of Constituents from Eclipta Prostrata L.

The study conducted three years ago aimed to identify the anti-diabetes and anti-obesity properties of the Eclipta prostrata L. plant. The researchers found that certain fatty acids (23, 24) from the plant exhibited potent protein tyrosine phosphatases (PTP)1B inhibitory effects, which are associated with diabetes and obesity, with IC50 values of 2.14 and 3.21 µM. Triterpenoid-glycosides (12–15) also showed strong to moderate PTP1B inhibitory effects, with IC50 values ranging from 10.88 to 53.35 µM. Additionally, certain compounds (1–4, 7, 8, 10) demonstrated significant anti-inflammatory activity by inhibiting nitric oxide production in lipopolysaccharide-stimulated RAW264.7 cells. Particularly, compound 9 showed potent inhibitory effects. Further analysis was performed to identify the inhibitory expression including heme oxygenase-1 (HO-1) and inhibitor of kappaB (IκB) phosphorylation.