Analysis of Proliferation, Apoptosis, and Keratin Expression in Cultured Normal and Immortalized Human Buccal Keratinocytes

    February 2003 in “ European Journal Of Oral Sciences ”
    Annette Hansson, Balvinder K. Bloor, Zsolt Sarang, Ylva Haig, Peter R. Morgan, Hans‐Jürgen Stark, Norbert E. Fusenig, Jan Ekstrand, Roland C. Grafström
    Studysummary This study found that the SVpgC2a keratinocyte cell line, used as a model for dysplastic epithelium, showed increased apoptosis, proliferation, and aberrant keratin expression compared to normal keratinocytes from buccal mucosa. Our plain-language summary of this paper — not a Tressless recommendation.
    The study analyzed the growth and differentiation functions of normal keratinocytes (NOK) and an SV40T-immortalized keratinocyte line (SVpgC2a) from buccal mucosa, using the latter as a model for dysplastic epithelium. It was found that SVpgC2a exhibited three- to five-fold higher apoptotic and proliferative activity compared to NOK. Serum conditions did not significantly affect NOK but supported SVpgC2a proliferation. Both cell types expressed basal lamina markers and vimentin, indicating a proliferative state. SVpgC2a showed reduced keratin expression, including the non-keratinizing marker K13, and transcribed keratins associated with epithelial dysplasia. The findings suggested aberrant apoptosis, proliferation, and keratin expression in SVpgC2a, modeling the progression from normal to pre-neoplastic buccal epithelium.
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