Proliferation and differentiation of organoid hair follicle cells co-cultured with fat cells in collagen gel matrix culture

July 1998 in “ British Journal of Dermatology ”

Misago, Toda, Sugihara, Kohda, Narisawa

The study investigated the effects of fat cells on the proliferation and differentiation of organoid hair follicle cells using a collagen gel matrix culture system with rat skin. Three conditions were tested: control with cell-free collagen gel, co-culture with fat cells in close apposition, and co-culture with fat cells in spatial separation. It was found that fat cells inhibited the proliferation of both organoid hair follicle cells and fibroblasts when in close contact, but accelerated the differentiation of hair follicle cells. This suggests that fat cells may have an inhibitory effect on fibroblast proliferation, which could maintain normal follicular structure and promote hair-type differentiation. The results indicated that either the concentration gradient of soluble factors from fat cells or direct contact between hair follicle cells and fat cells influenced these outcomes.

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