Perifollicular Vascularization: Cocultures and Tricultures Approaches to Study the Cross-Talk Between Microvascular Endothelial Cells, Follicle Papilla Cells, and Keratinocytes
December 2015
in “
Vascular Pharmacology
”
follicle dermal papilla cells human microvascular endothelial cells keratinocytes hair follicle vascularization hair growth hair loss androgenetic alopecia dermal fibroblasts proangiogenic growth factors VEGF IGF-1 tri-culture system conditioned medium proliferation of follicular keratinocytes metabolic factors inflammatory cytokines vascular remodeling FDPCs HMVECs AA vascular growth factors
TLDR Hair papilla cells are crucial for blood vessel development in hair follicles, affecting hair growth and loss.
The study from 2015 investigated the interaction between follicle dermal papilla cells (FDPCs), human microvascular endothelial cells (HMVECs), and keratinocytes in vitro to understand their role in hair follicle vascularization and potential implications for hair growth and hair loss conditions like Androgenetic Alopecia. The findings showed that FDPCs greatly enhanced the survival, growth, and tube formation of HMVECs, surpassing the effects of dermal fibroblasts. FDPCs also increased the release of proangiogenic growth factors such as VEGF and IGF-1. In a tri-culture system, the conditioned medium stimulated the proliferation of follicular keratinocytes and influenced the expression of metabolic factors and inflammatory cytokines. The study concluded that papilla cells are key in the vascular remodeling of the hair follicle, which is important for understanding the mechanisms behind hair growth and loss.
