In Vivo Non-Invasive Observation of Dynamic Changes in Hair Shaft, Melanin, and Collagen During the C57BL/6 Follicle Cycle
September 2025
in “
bioRxiv (Cold Spring Harbor Laboratory)
”
New to collagen? There is a guide in the encyclopedia. Read the guide → 
Studysummary In this study, researchers developed non-invasive methods to monitor hair shaft, melanin, and collagen dynamics in C57BL/6 mice across different hair follicle cycle phases, supporting non-invasive research in hair loss treatment development. Our plain-language summary of this paper — not a Tressless recommendation.
This study utilized non-invasive methods to monitor the dynamics of hair shaft, melanin, and collagen in C57BL/6 mice throughout multiple hair follicle cycles. Techniques such as dermoscopy, photography, electron microscopy, and second harmonic generation (SHG) were employed to observe and quantify changes during the anagen, catagen, and telogen phases. The accuracy of these observations was validated using hematoxylin and eosin staining, liquid crystal polarizing imaging, and Sirius Red staining. The research provides a foundation for non-invasive evaluation of hair growth cycles, which could aid in developing treatments for hair loss. The authors reported no competing interests.
